Evaluation of the Anti-biofilm Effect of a New Anti-bacterial Silver Citrate/Lecithin Coating in an In-vitro Experimental System Using a Modified Robbins Device

Evaluation of the Anti-biofilm Effect of a New Anti-bacterial Silver Citrate/Lecithin Coating in an In-vitro Experimental System Using a Modified Robbins Device

Hideaki HASHIMOTO
Department of Urology, Okayama University School of Medicine

感染症学雑誌　2001 年 75 巻 8 号 p. 678-685

【Abstract】

We examined the anti-biofilm effect of a new antibacterial coating catheter material composed of a mixture of silver citrate, lecithin, and liquid silicone in a 2:2:8 ratio using an in vitro experimental system with a modified Robbins device. The bacterial adhesion inhibition effect, growth inhibition effect on attached bacteria, and the persistence of these effects were observed using two strains of Pseudomonas aeruginosa and one strain of Escherichia coli. Strong bacterial adhesion inhibition effects were observed in one strain of P. aeruginosa and E. coli. In contrast to silicone, which formed mature biofilms, the antibacterial catheter material only exhibited scattered bacterial adhesion in the highly biofilm-forming P. aeruginosa strain 14-210. Furthermore, the P. aeruginosa strain 14-210, which once adhered to the surface, was inhibited in its growth by silver ions eluted from the antibacterial catheter material. Moreover, these anti-biofilm effects did not diminish even after at least 48 hours of artificial urine exposure.

Robbins device

In the antibacterial test of the prototype catheter segments, the antibacterial effect against E. coli persisted even after 3 and 7 days of artificial urine treatment. Furthermore, the measurement of the eluted silver concentration from the prototype catheter showed approximately 200 ppb of silver concentration even after 7 days. Based on this, it is believed that the effect can be expected to last for at least around 7 days.

Biofilm Reference photo