Evaluation of anti-thrombotic efficacy of newly developed catheter coated with urokinase together with heparin
ウロキナーゼ，ヘパリン併用被覆カテーテルの血栓形成阻害効果の検討
MIKAMI HIDETOMO (日本シャーウッド、浜松医大 生理学第2) , KOBAYASHI TAKAO (信州大 医 保健学科) , URANO TETSUMEI (浜松医大 生理学第2)
We developed an antithrombotic catheter coated with both urokinase and heparin on its surface and evaluated its performance in vitro. Using a modified Chandler Loop method with human whole blood, the catheter demonstrated higher antithrombotic properties compared to those coated with urokinase or heparin alone, and these properties were maintained even after a 25-day rinse period. TAT and D-dimer levels in the blood within the loop increased with the extended rinse period, with a significant rise in D-dimer levels following the increase in TAT. Measurements of urokinase and heparin activity on the catheter surface, using synthetic substrates S-2251 and S-2238 for plasmin and thrombin, respectively, confirmed that both activities were retained even after the 25-day rinse period. It was revealed that coating the catheter surface with both heparin and urokinase resulted in the manifestation of two distinct antithrombotic actions—anticoagulant and fibrinolytic activation—thus maintaining long-term antithrombotic properties.
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