Studies on Antithrombogenicity of Urokinase Immobilized Polymer

1988 年 108 巻 1 号 p. 44-49

By taking notice of the fibrinolytic activity of urokinase (UK) which is a plasminogen activator, an antithrombogenic material was developed by immobilizing the UK on a material surface. That is, the immobilized UK possesses an action to transform catalytically the plasminogen in the blood into plasmin, and exhibits its antithrombotic action by dissolving constantly the fibrins produced on the material surface. The UK immobilized on nylon and ethylene vinyl acetate copolymer (EVA) surfaces showed stable activity both in vitro and in vivo. Thrombus formation time (TFT) of the UK immobilized materials was remarkably longer than that of non-treated materials. Since UK inhibiting substances were reported to be present in the blood, a nitrophthalic acid having an affinity for the UK inhibiting substances was co-immobilized with UK to see whether the inhibitory effect of UK could be prevented or not. The materials co-immobilized with UK and the nitrophthalic acid showed superior stability compared to that without the nitrophthalic acid. The UK immobilized material proved to be an adequate antithrombogenic material.

HCl;3N HCl 30℃　30min

NaOH;15%(W/W)NaOH in 20% water in methanol　50℃　2h

Gantrez;4% Gantrez in anhydrous acetone, ambient temp 2.5h

※Conditions for use as a simple surface modification.